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Updated: Oct 17, 2025

Conducting Multiple Imaging Modes with One Fluorescence Microscope

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Published on: October 28, 2018

The Lightfield Microscope Eyepiece.

Nicolò Incardona^1,2, Ángel Tolosa^1,2, Gabriele Scrofani¹

¹3D Imaging and Display Laboratory, Department of Optics, Universitat de València, 46100 Burjassot, Spain.

Sensors (Basel, Switzerland)

|October 13, 2021

Summary

This summary is machine-generated.

A new lightfield microscope eyepiece converts standard microscopes into 3D imaging devices. This innovation enables high-speed, three-dimensional imaging for various scientific applications.

Keywords:

3D microscopy FiMic Fourier integral microscope lightfield eyepiece lightfield microscopy plenoptic eyepiece

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Last Updated: Oct 17, 2025

Conducting Multiple Imaging Modes with One Fluorescence Microscope

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Published on: October 28, 2018

High-resolution Fiber-optic Microendoscopy for in situ Cellular Imaging

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Published on: January 11, 2011

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope

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Published on: August 15, 2014

Area of Science:

Optics and Photonics
Microscopy
Biomedical Imaging

Background:

Lightfield microscopy offers rapid 3D imaging capabilities crucial for time-resolved applications.
Conventional microscopes lack the inherent ability to capture volumetric data in a single shot.

Purpose of the Study:

To develop a novel, adaptable device that transforms any conventional microscope into a functional lightfield microscope.
To enable 3D reconstruction of samples using existing microscope infrastructure.

Main Methods:

Design of a lightfield microscope eyepiece based on the Fourier integral microscope concept.
Integration of the eyepiece with standard microscope components (objectives, illumination, stage).
Construction of a proof-of-concept device using readily available optomechanical parts.

Main Results:

Demonstration of the optical performance of the lightfield eyepiece, aligning with theoretical predictions.
Acquisition of 3D images of various samples using the developed eyepiece.
Successful 3D reconstruction of samples, validating the device's functionality.

Conclusions:

The designed lightfield microscope eyepiece effectively enables 3D imaging with conventional microscopes.
The proof-of-concept device validates the feasibility of converting existing microscopes for lightfield applications.
This work establishes a foundation for a commercially viable device compatible with diverse microscopes.