NAD-seq for profiling the NAD⁺ capped transcriptome of Arabidopsis thaliana

Eukaryotic RNAs can be modified with a non-canonical 5' nicotinamide adenine dinucleotide (NAD⁺) cap. NAD-seq identifies transcriptome-wide NAD⁺ capped RNAs. NAD-seq takes advantage of click chemistry to allow the capture of NAD⁺ capped RNAs. Unlike other approaches, NAD-seq does not require DNA synthesis on beads, but this technique uses full NAD⁺ capped transcripts eluted from beads as the substrates for strand-specific RNA sequencing library preparation. For complete details on the use and execution of this protocol, please refer to Yu et al. (2021).