Hydrogenotrophic methanogenic granular sludge formation for highly efficient transforming hydrogen to CH₄

This paper presents a potential process that can enhance H₂ transformation to CH₄ and simultaneously upgrading biogas by using hydrogenotrophic methanogens. For the first time, anaerobic granules were developed in upflow anaerobic sludge blanket (UASB) reactor feeding H₂/CO₂ syngas as the sole substrate and the granule characterization was thoroughly investigated. The results from experiment revealed that the H₂ consumption rates of UASB reactor increased from 32.2 mmol L^-1·d^-1 at H₂ feeding rate 0.08 g L^-1·d^-1 to 132.0 mmol L^-1·d^-1 at 0.37 g L^-1·d^-1, indicating that the hydrogenotrophic methanogenesis pathway was stimulated by injection of H₂. Abundant cavities and cracks were observed on the surface and cross-section of granules, which greatly facilitated internally transferring H₂/CO₂ synthesis gas and biogas escape. The abundance of hydrogenotrophic Methanobacterium increased, while Methanosaeta, Methanosarcina, and Methanomassiliicoccus decreased with increasing H₂ feeding rate. In general, this paper offers a feasible solution in terms of energy transformation and connecting power to fuel.