Hemodynamic Mimic Shear Stress for Platelet Membrane Nanobubbles Preparation and Integrin α_IIbβ₃ Conformation...

Area of Science:

• Biomaterials Science
• Nanotechnology
• Biomedical Engineering

Background:

• Platelet (PLT) membrane biomimetic nanomaterials offer theranostic potential due to biocompatibility.
• Current methods lack precise control over biomimetic nanomaterial component assembly.
• Understanding platelet production and activation dynamics is key for novel nanomaterial design.

Purpose of the Study:

• To develop a controllable method for fabricating platelet nanobubbles (PNBs) with reassembled platelet membranes.
• To investigate the effect of shear stress on platelet membrane component organization and integrin conformation.
• To evaluate the potential of PNBs for targeted ultrasound diagnosis of vascular endothelial injury.

Main Methods:

• Fabrication of PNBs using an adjustable pressure-induced shear stress method.
• Analysis of platelet membrane lipid rafts and protein enrichment under high shear stress.
• Assessment of the conformation change in platelet integrin α_IIbβ₃.
• Evaluation of PNB adhesion to vascular endothelial injury models.

Main Results:

• Adjustable pressure-induced shear stress successfully fabricated PNBs with reassembled platelet membranes and ideal echogenicity.
• High shear stress enriched platelet membrane lipid rafts and proteins on the gas-liquid interface.
• Platelet integrin α_IIbβ₃ adopted a shear stress-induced intermediate affinity state, enhancing adhesion.
• PNBs demonstrated improved adhesion to vascular endothelial injury sites.

Conclusions:

• The developed shear stress method provides controllable construction of platelet membrane biomimetic nanomaterials.
• PNBs exhibit enhanced targeting capabilities for vascular endothelial injury due to altered integrin conformation.
• PNBs hold significant promise for targeted ultrasound diagnosis in vascular applications.