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Tracking neuronal motility in live murine retinal explants.
Nozie D Aghaizu1, Katherine M Warre-Cornish1, Martha R Robinson1
1University College London, Institute of Ophthalmology, London EC1V 9EL, UK.
STAR Protocols
|December 17, 2021
Summary
This study introduces a new protocol to track cell movement within live mouse retinae, crucial for understanding retinal development and diseases. The method is adaptable for various cell types and imaging models.
Area of Science:
- Developmental biology
- Neuroscience
- Ophthalmology
Background:
- The developing retina exhibits dynamic cellular reorganization.
- Intra-retinal cell motility is a key aspect of retinal development.
Purpose of the Study:
- To present a protocol for tracking retinal cell motility in live explanted mouse retinae.
- To provide a method applicable to various cell types and retinal models.
Main Methods:
- Live imaging of explanted mouse retinae.
- Focus on careful tissue handling for high-quality data acquisition.
- Semi-automated in silico data handling procedures.
Main Results:
- A detailed protocol for tracking retinal cell motility is established.
- The protocol is demonstrated for rod and cone photoreceptors.
- The method's applicability to other fluorescently labeled cells is confirmed.
Conclusions:
- The presented protocol enables robust tracking of retinal cell motility.
- This method supports research into retinal development and disease mechanisms.
- The protocol is versatile for different cell types and experimental models.
