Related Experiment Video
Updated: Oct 7, 2025
09:10
An Intra-Tissue Radiometry Microprobe for Measuring Radiance In Situ in Living Tissue
Published on: June 2, 2023
754
Optical property recovery with spatially-resolved diffuse reflectance at short source-detector separations using a
Karina G Bridger1, Jacob R Roccabruna1, Timothy M Baran1,2
1Department of Biomedical Engineering, University of Rochester, 201 Robert B. Goergen Hall, P.O. Box 270168, Rochester, NY 14627, USA.
Biomedical Optics Express
|January 10, 2022
Summary
A new fiber-optic probe accurately measures tissue optical properties using diffuse reflectance. This technology aids in developing personalized photodynamic therapy treatment plans for conditions like human abscesses.
Area of Science:
- Biomedical Optics
- Medical Imaging
- Photodynamic Therapy
Background:
- Accurate tissue optical property determination is crucial for effective photodynamic therapy (PDT).
- Existing methods for optical property recovery can be complex or invasive.
Purpose of the Study:
- To develop and validate a compact fiber-optic probe for spatially-resolved diffuse reflectance measurements.
- To enable accurate recovery of tissue optical properties, specifically scattering and absorption coefficients.
- To lay the groundwork for clinical application in guiding PDT for human abscesses.
Main Methods:
- A compact fiber-optic probe (2 mm outside diameter) was designed for diffuse reflectance spectroscopy.
- Phantom studies were conducted using Intralipid 20% as a scatterer and methylene blue, MnTPPS, and India ink as absorbers.
- Spatially-resolved diffuse reflectance measurements were analyzed to recover optical properties.
Main Results:
- The reduced scattering coefficient was recovered with a root mean square error (RMSE) of 0.86–2.7 cm⁻¹ (average error 3.8%).
- Methylene blue concentration was recovered with RMSE of 0.26–0.52 µM (average error 15.0%), unaffected by MnTPPS presence.
- The system demonstrated reliable performance across various optical property conditions.
Conclusions:
- The developed fiber-optic probe system accurately recovers tissue optical properties.
- This technology shows promise for non-invasive assessment of optical properties in clinical settings.
- The system will be applied to human abscesses to optimize photodynamic therapy planning.
