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Updated: Oct 4, 2025

Assessing Autophagic Flux by Measuring LC3, p62, and LAMP1 Co-localization Using Multispectral Imaging Flow Cytometry
Published on: July 21, 2017
An indole-based fluorescent chemosensor targeting the autophagosome
Sun Young Park1, Kyutae Kim2, Dong-Hyung Cho3
1Department of Chemistry, Sookmyung Women's University, Seoul 04310, Korea. minheelee@sookmyung.ac.kr.
Abstract:
Autophagy is a process for the degradation and recycling of intracellular components and dysfunctional organelles. We developed an indole-embedded fluorescent naphthalimide for the selective imaging of autophagosomes in live cells. It was shown as intense puncta in the fluorescence confocal images and co-localizes with an autophagosome marker, LC3-RFP. In addition, it was applied to cellular autophagic models based on ER stress and starvation to verify its capability.

