Crystallization and preliminary characterization of CheY, a chemotaxis control protein from Escherichia coli

Single crystals of the 14.1-kDa cheY gene product from Escherichia coli have been grown from buffered ammonium sulfate solutions using the combined methods of microdialysis and pulsed diffusion. The crystals are of the monoclinic space group P2(l), have cell constants of a = 51.4 A, b = 112 A, c = 51.2 A, and beta = 107.3 degrees, and contain four molecules per asymmetric unit. They are stable to x-ray radiation and diffract beyond 3.0 A resolution.