Oxidative Stress Modulation by Carnosine in Scaffold Free Human Dermis Spheroids Model: A Proteomic Study

Area of Science:

• Biochemistry
• Dermatology
• Proteomics

Background:

• Carnosine is a dipeptide with antioxidant and anti-aging properties.
• Understanding carnosine's mechanism in human skin aging is crucial for developing effective skincare.
• Previous studies lacked in-depth proteomic analysis of carnosine's effects on skin aging models.

Purpose of the Study:

• To investigate the mechanism of action of carnosine on the human skin proteome.
• To characterize the aging process in a 3D dermal fibroblast spheroid model.
• To evaluate carnosine's potential to prevent age-related changes in skin.

Main Methods:

• Utilized a 3D scaffold-free spheroid model of primary dermal fibroblasts.
• Employed label-free quantitative proteomics with high-resolution mass spectrometry.
• Integrated network analyses to identify modulated biological pathways.

Main Results:

• Characterized 2171 quantified proteins in the 3D dermis model over 14 days.
• Identified 485 differentially regulated proteins upon carnosine treatment at 7 days.
• Observed carnosine's modulation of mitochondrial function, oxidative stress, and extracellular matrix reorganization, counteracting aging-induced pathways.

Conclusions:

• Carnosine treatment downregulates oxidative stress pathways, preventing the aging process in dermis spheroids.
• Quantitative proteomics on 3D spheroid models offers insights into skin aging mechanisms.
• This approach holds promise for personalized therapies and novel skincare strategies.