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Orthogonal Multiplexed NIR-II Imaging with Excitation-Selective Lanthanide-Based Nanoparticles.
Houben Xu1, Yang Yang1, Lingfei Lu1
1Department of Chemistry, State Key Laboratory of Molecular Engineering of Polymers, Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials and iChem, Fudan University, Shanghai 200433, People's Republic of China.
Analytical Chemistry
|February 17, 2022
Summary
Researchers developed novel nanoparticles for multiplexed imaging in the second near-infrared (NIR-II) window. These excitation-selective down-shifting nanoparticles (DSNPs) enable distinct signals for advanced biological studies and anticounterfeiting.
Area of Science:
- Biomedical Optics
- Materials Science
- Nanotechnology
Background:
- Multiplexed imaging in the second near-infrared (NIR-II) window offers reduced scattering and background noise for biological studies.
- Developing probes with distinct emission profiles for multiplexing in the NIR-II window remains a challenge.
- Existing probes lack separated signals, limiting in vivo multiplexed imaging potential.
Purpose of the Study:
- To design and synthesize novel excitation-selective down-shifting nanoparticles (DSNPs) for multiplexed imaging in the NIR-II window.
- To achieve orthogonal, three-color emissions in the NIR-II window using distinct excitation wavelengths.
- To demonstrate the potential of these DSNPs in anticounterfeiting and anatomical imaging applications.
Main Methods:
- Synthesized core-shell structured DSNPs using Nd3+, Ho3+, and Er3+ ions (csNd, csHo, csEr).
- Screened and confirmed excitation wavelengths at 730, 915, and 655 nm for the DSNPs.
- Evaluated DSNP performance in encrypted anticounterfeiting and in vivo anatomical imaging of mouse tissues.
Main Results:
- Achieved efficient, orthogonal three-color emissions in the NIR-II window (1060, 1180, 1525 nm) with excitation-selective DSNPs.
- Demonstrated successful encrypted anticounterfeiting with increased optical codes.
- Successfully performed anatomical rotation imaging in mice, differentiating tissues with high contrast and resolution by switching excitation wavelengths.
Conclusions:
- The designed NIR-II excitation-selective DSNPs provide a versatile platform for multiplexed imaging.
- These DSNPs offer a powerful framework for advanced biological studies and accurate diagnosis.
- The technology shows promise for anticounterfeiting and high-resolution in vivo imaging applications.
