Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

8.8K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
8.8K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

15.7K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
15.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Pharmacokinetics, pharmacodynamics, and safety interaction study of tunodafil hydrochloride and alcohol: A randomized, blinded, placebo-controlled, three-cycle crossover study in Chinese healthy men.

Andrology·2025
Same author

Near-infrared light activatable chemically induced CRISPR system.

Light, science & applications·2025
Same author

A self-gelling hemostatic powder driven by hydrogen bonding and electrostatic interactions with antibacterial and antioxidant properties.

Journal of materials chemistry. B·2025
Same author

3D Printing Continuous Fiber Reinforced Polymers: A Review of Material Selection, Process, and Mechanics-Function Integration for Targeted Applications.

Polymers·2025
Same author

<i>Helicobacter Pylori</i>-Induced Apoptosis in Gastric Diseases: Mechanisms, Implications, and Diagnostic Applications.

International journal of general medicine·2025
Same author

[Study on the promotion of podocyte pyroptosis by high glucose-stimulated GMC-derived exosomes and the intervention effects of Tongluo Yishen Formula].

Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology·2025

Related Experiment Video

Updated: Oct 2, 2025

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
12:51

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

9.0K

Calibration method for an extended depth-of-field microscopic structured light system.

Liming Chen, Xiaowei Hu, Song Zhang

    Optics Express
    |February 24, 2022
    PubMed
    Summary

    This study introduces a new calibration method for microscopic structured light systems, enhancing depth of field (DOF) measurement accuracy to 1.0 μm. The technique corrects phase errors for precise 3D reconstruction in extended DOF applications.

    More Related Videos

    Measuring Spatially- and Directionally-varying Light Scattering from Biological Material
    11:57

    Measuring Spatially- and Directionally-varying Light Scattering from Biological Material

    Published on: May 20, 2013

    13.7K
    A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
    11:15

    A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

    Published on: May 30, 2016

    25.5K

    Related Experiment Videos

    Last Updated: Oct 2, 2025

    Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
    12:51

    Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

    Published on: December 9, 2013

    9.0K
    Measuring Spatially- and Directionally-varying Light Scattering from Biological Material
    11:57

    Measuring Spatially- and Directionally-varying Light Scattering from Biological Material

    Published on: May 20, 2013

    13.7K
    A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
    11:15

    A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

    Published on: May 30, 2016

    25.5K

    Area of Science:

    • Metrology
    • Optical Engineering
    • Microscopy

    Background:

    • Microscopic structured light systems require accurate calibration for precise 3D measurements.
    • Extended depth of field (DOF) is crucial for capturing complex micro-topographies.
    • Phase errors in calibration targets can limit measurement accuracy.

    Purpose of the Study:

    • To develop an improved calibration method for microscopic structured light systems.
    • To extend the depth of field (DOF) measurement range.
    • To enhance the accuracy of 3D measurements by mitigating calibration-induced phase errors.

    Main Methods:

    • Utilized the focal sweep technique to achieve an extended depth of field (DOF).
    • Developed a computational framework incorporating a polynomial interpolation algorithm.
    • Corrected phase errors near calibration target features for improved phase map generation.

    Main Results:

    • Achieved a measurement accuracy of approximately 1.0 μm.
    • Demonstrated accurate 3D measurements within a volume of 2,500 μm (W) × 2,000 μm (H) × 500 μm (D).
    • Successfully alleviated the impact of phase errors from standard calibration targets.

    Conclusions:

    • The proposed calibration method enhances the accuracy of microscopic structured light systems.
    • The technique enables precise 3D measurements over an extended depth of field (DOF).
    • This advancement is valuable for high-accuracy micro-metrology applications.