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Updated: Oct 1, 2025
Single-Cell Optical Action Potential Measurement in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes
Published on: December 22, 2020
A universal, multimodal cell-based biosensing platform for optimal intracellular action potential recording.
Dongxin Xu1, Jiaru Fang1, Moran Yadid2
1State Key Laboratory of Optoelectronic Materials and Technologies, Guangdong Province Key Laboratory of Display Material and Technology, School of Electronics and Information Technology, Sun Yat-sen University, Guangzhou, 510006, China.
This study introduces a new biosensing platform for consistent, high-quality intracellular recordings using tunable electroporation. The advanced system facilitates facile, low-cost electrophysiological studies in excitable cells.
Area of Science:
- Biomedical Engineering
- Electrophysiology
- Cellular Biology
Background:
- Intracellular recording of action potentials is crucial for studying disease mechanisms in excitable cells like cardiomyocytes and neurons.
- Current methods, such as 3D nanoelectrodes and transient electroporation, face challenges including complex fabrication, high costs, and inconsistent results, limiting high-throughput applications.
Purpose of the Study:
- To develop an advanced, cell-based biosensing platform for consistent and high-quality intracellular recordings.
- To enable tunable electroporation with controllable parameters and real-time feedback for improved electrophysiological studies.
Main Methods:
- Development of a universal cell-based biosensing platform integrating with electrode arrays.
- Implementation of tunable electroporation with controllable pulse parameters (width, number, amplitude).
- Real-time analysis of recorded potentials for instantaneous feedback on electroporation effectiveness.
Main Results:
- Demonstrated effective electroporation and high-quality intracellular recordings through tuning of electroporation parameters.
- The platform enables facile, stable, and reliable intracellular recording.
- The system utilizes microelectrode arrays suitable for large-scale production and low-cost modules.
Conclusions:
- The proposed integrated platform offers a facile, low-cost, and repeatable solution for electrophysiological studies.
- This technology has the potential to significantly advance large-scale, convenient intracellular recording.
- The tunable electroporation system overcomes limitations of existing methods, improving throughput and reliability.
