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Updated: Oct 1, 2025

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Corneal Donor Tissue Preparation for Descemet's Membrane Endothelial Keratoplasty
Published on: September 17, 2014
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Safety-Net Suture for Aphakic Descemet Membrane Endothelial Keratoplasty
Osvaldo Berger1, Jaime Kriman1, Alfonso Vasquez-Perez1
1The Cornea and External Disease Service, Moorfields Eye Hospital NHS Foundation Trust, London, United Kingdom; and.
Cornea
|March 7, 2022
Summary
A novel "safety-net suture" technique effectively prevents donor dislocation during Descemet membrane endothelial keratoplasty (DMEK) in complex unicameral eyes. This simple, low-cost method enhances surgical outcomes for aphakic patients with large iris defects.
Area of Science:
- Ophthalmology
- Corneal Surgery
- Surgical Innovation
Background:
- Descemet membrane endothelial keratoplasty (DMEK) is a corneal transplantation technique.
- Aphakic eyes with large iris defects (unicameral eyes) present unique challenges for DMEK, increasing the risk of donor dislocation.
Purpose of the Study:
- To describe a novel, low-cost, and accessible surgical technique, the "safety-net suture," to mitigate the risk of posterior donor dislocation during DMEK in unicameral eyes.
Main Methods:
- A retrospective review of 3 cases of aphakic bullous keratopathy in unicameral eyes undergoing DMEK.
- Implementation of the safety-net suture technique: a continuous 10-0 polypropylene suture placed in a cat's-cradle pattern anterior to the trabecular meshwork to create a temporary anterior chamber barrier.
- Suture removal after air tamponade of the DMEK donor graft.
Main Results:
- Successful completion of DMEK in all 3 cases without intraoperative posterior donor dislocation.
- One case experienced partial postoperative graft detachment, successfully managed with repeat air tamponade.
- Corneal clarity maintained in early follow-up (1-10 months post-surgery).
Conclusions:
- The safety-net suture is a simple, cost-effective method for reducing the risk of intraoperative posterior donor dislocation in DMEK procedures on unicameral, aphakic eyes.
- This technique offers a valuable solution for complex cases, improving surgical safety and graft stability.

