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Related Experiment Video

Updated: Sep 30, 2025

Author Spotlight: Studying Host-Virus Interactions with Pseudotyped Viruses

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Published on: November 21, 2023

A decoy mutant ACE2 designed to reduce COVID-19.

Mazharul Maishan¹, Diana L Lim², Guy A Zimmerman³

¹Cardiovascular Research Institute, University of California at San Francisco, San Francisco, CA, USA.

Trends in Pharmacological Sciences

|March 14, 2022

Summary

A novel mutant angiotensin-converting enzyme 2 (ACE2) protein was engineered to combat COVID-19. This therapeutic strategy effectively reduces viral uptake and lung injury caused by SARS-CoV-2 infection.

Keywords:

ARDS COVID-19 therapy viral pneumonia

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Area of Science:

Biochemistry
Virology
Drug Discovery

Background:

The ongoing emergence of SARS-CoV-2 variants necessitates the development of new therapeutic strategies for COVID-19.
Current treatments face challenges due to viral evolution and the need for effective interventions.

Purpose of the Study:

To engineer a mutant angiotensin-converting enzyme 2 (ACE2) with therapeutic potential against SARS-CoV-2.
To evaluate the efficacy of the engineered ACE2 in blocking viral entry and mitigating lung injury.

Main Methods:

Site-directed mutagenesis was used to create a modified ACE2 protein.
The mutant ACE2 was tested for its binding affinity to the SARS-CoV-2 spike protein.
Viral uptake assays were performed using human lung cells.
In vivo studies were conducted in mice expressing human ACE2 to assess lung injury.

Main Results:

The engineered mutant ACE2 demonstrated competitive binding to the SARS-CoV-2 spike protein.
A significant reduction in viral uptake by human lung cells was observed.
Amelioration of SARS-CoV-2-induced lung injury was confirmed in a mouse model.

Conclusions:

Engineered mutant ACE2 represents a promising therapeutic candidate for COVID-19.
This approach offers a novel strategy to neutralize SARS-CoV-2 and protect lung tissue.
Further research is warranted to explore its clinical applicability.