[2-cycle culture of Vibrio cholerae in a fermenter]

The method for the two-cycle cultivation of V. cholerae, reference strains Inaba and Ogawa, in a fermenter has been developed. In the first and second cultivation cycles no difference in the yield of the biomass and in the ultrastructure of the vibrios has been established. In the second cycle a decrease in the minimum time of generation has been observed. The vaccines obtained in the first and second cultivation cycles have not been found to differ in their antigenic and immunogenic activity. The vaccines meet the WHO requirements.