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O-GlcNAcylation regulates lysophosphatidic acid-induced cell migration by regulating ERM family proteins

Minseok Song1, Pann-Ghill Suh2

  • 1Department of Life Sciences, Yeungnam University, Gyeongsan, South Korea.

FEBS Open Bio
|March 29, 2022
PubMed

Insights

O-GlcNAcylation (O-GlcNAc) regulates ovarian cancer cell migration by affecting ERM proteins. Increased O-GlcNAc reduces cell migration and membrane protrusion, suggesting a role in metastasis.

Area of Science:

  • Biochemistry
  • Cell Biology
  • Cancer Research

Background:

  • O-GlcNAcylation (O-GlcNAc) is a post-translational modification that competes with phosphorylation in cell signaling.
  • O-GlcNAc plays a role in cancer metastasis.

Purpose of the Study:

  • To investigate the role of O-GlcNAc in lysophosphatidic acid (LPA)-induced ovarian cancer cell (OVCAR-3) migration.
  • To explore the relationship between O-GlcNAc and ERM protein modification in OVCAR-3 cells.

Main Methods:

  • Studied O-GlcNAc and phosphorylation levels of ERM proteins in OVCAR-3 cells.
  • Assessed the impact of O-GlcNAc accumulation on LPA-induced OVCAR-3 cell migration and membrane protrusion formation.

Main Results:

  • O-GlcNAc modification of ERM proteins was inversely correlated with their phosphorylation.
  • Accumulation of O-GlcNAc reduced LPA-induced membrane protrusion and OVCAR-3 cell migration.

Conclusions:

  • O-GlcNAc is a key regulator of OVCAR-3 cell migration.
  • O-GlcNAc influences ERM protein function, impacting cell motility and metastasis potential.
Keywords:
ERM family proteinsO-GlcNAcylationlysophosphatidic acidmigrationovarian cancer

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