Related Experiment Video
Updated: Sep 28, 2025

Loading a Calcium Dye into Frog Nerve Endings Through the Nerve Stump: Calcium Transient Registration in the Frog Neuromuscular Junction
Published on: July 8, 2017
Role of α7 Nicotinic Acetylcholine Receptors in Synaptic Transmission in Frog Neuromuscular Contacts
O A Lenina1, I V Kovyazina2,3
1A. E. Arbuzov Institute of Organic and Physical Chemistry, Federal Research Center Kazan Scientific Center, Russian Academy of Sciences, Kazan, Republic of Tatarstan, Russia.
Abstract:
The role of α7 nicotinic acetylcholine receptors in coupling of synaptic activity and muscle contractions was studied in frog (Rana ridibunda) neuromuscular synapses. The amplitude of endplate currents, the probability of action potential generation, and the strength of muscle contractions decreased in the presence of selective α7 antagonist methyllycaconitine. The effects of nicotinic acetylcholine receptor blockade depended on the pattern of the motor nerve stimulation. It can be assumed that the muscle action potential is a factor of retrograde control of neurosecretion, which modulates activity of α7 nicotinic receptors and the release of acetylcholine from motor nerve endings.
More Related Videos
10:45Levator Auris Longus Preparation for Examination of Mammalian Neuromuscular Transmission Under Voltage Clamp Conditions
Published on: May 5, 2018
12:19Live Imaging of Nicotine Induced Calcium Signaling and Neurotransmitter Release Along Ventral Hippocampal Axons
Published on: June 24, 2015
Related Concept Videos
Neuromuscular Junction And Blockade
Cholinergic Receptors: Nicotinic
There are two types of nicotinic receptors: neuromuscular (NM/NM/N1) and neuronal (NN/NN/N2). The two families differ based on their location and selectivity to...
Cholinergic Receptors: Muscarinic
The subtypes M1, M3, and M5 couple with the Gq subunit and activate the phospholipase C (PLC) activity, mobilizing intracellular Ca2+....
Cholinergic Neurons: Neurotransmission
The Neuromuscular Junction
Neurochemical Transmission: Sites of Drug Action