A simplified technique for rapid immunofluorescent assessment of cellular hormonal content

Currently applied techniques for immunofluorescent staining of cultured cells are time consuming and not amenable to experimental design. Monolayers of rat anterior pituitary cells were stained in situ with rabbit follicle-stimulating hormone antiserum. Excision of the culture surfaces produced samples that were easy to handle and score for immunoreactivity without sacrificing resolution. The staining of in situ monolayers of cultured cells allows for assessment of both the secretory behavior and the hormonal content of the cells.