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Published on: December 3, 2011
An undergraduate laboratory to detect viruses in human DNA samples using qPCR
Kevin T Militello1, Hristina Nedelkovska1
1Biology Department, State University of New York at Geneseo, Geneseo, New York, USA.
Summary
This undergraduate lab teaches students quantitative PCR (qPCR) for viral genome detection. Students successfully identified a viral genome in mock samples, demonstrating effective qPCR learning.
Area of Science:
- Biotechnology
- Molecular Biology
- Virology
Background:
- The COVID-19 pandemic highlighted the need for accurate viral detection methods.
- Quantitative Polymerase Chain Reaction (qPCR) is the gold standard for detecting viral genomes in clinical samples.
- Understanding qPCR principles, controls, and data interpretation is crucial for students.
Purpose of the Study:
- To describe a 2-week undergraduate laboratory exercise for detecting viral genomes using qPCR.
- To provide students with hands-on experience simulating a SARS-CoV-2 qPCR test.
- To assess student comprehension of viral qPCR testing through various metrics.
Main Methods:
- Students used qPCR to detect a viral genome (bacteriophage lambda) in mock human DNA samples.
- A variety of control samples were incorporated into the experimental design.
- Patient pooling strategies were implemented to mimic real-world diagnostic scenarios.
Main Results:
- The laboratory exercise demonstrated high rates of student success in detecting viral genomes.
- Pre- and post-quiz assessments indicated improved student understanding of viral qPCR testing.
- Student feedback was positive, affirming the educational value of the exercise.
Conclusions:
- The described laboratory effectively teaches undergraduate students the principles and application of qPCR for viral detection.
- This hands-on experience enhances students' ability to interpret qPCR data and understand critical controls.
- The exercise provides a valuable model for molecular diagnostics education in a post-pandemic world.
