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Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1
Published on: March 13, 2018
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Droplet microfluidic-based loop-mediated isothermal amplification (dLAMP) for simultaneous quantification of multiple
Ya-Ling Tan1,2, Tao Wang2, Jianjun He2
1BGI-ShenZhen, Shenzhen 518103, China.
STAR Protocols
|May 2, 2022
Summary
This study introduces a digital quantification protocol using droplet microfluidics-based loop-mediated isothermal amplification (dLAMP) for precise measurement of multiple nucleic acid targets. This method enhances accuracy in experimental and clinical diagnostics.
Area of Science:
- Molecular Biology
- Biotechnology
- Analytical Chemistry
Background:
- Accurate quantification of nucleic acids is crucial for diagnostics.
- Existing methods may lack specificity or scalability for multiple targets.
Purpose of the Study:
- To present a protocol for digital quantification of multiple nucleic acid targets.
- To establish a versatile platform for absolute quantification in various diagnostic settings.
Main Methods:
- Utilizing droplet microfluidics for sample compartmentalization.
- Employing loop-mediated isothermal amplification (LAMP) for sensitive detection.
- Implementing digital counting for absolute quantification of nucleic acid targets.
Main Results:
- Demonstrated high specificity in quantifying multiple nucleic acid targets.
- Established a robust platform for absolute quantification.
- Protocol is adaptable for diverse in vitro diagnostic applications.
Conclusions:
- The dLAMP protocol offers a precise and adaptable solution for multiplex nucleic acid quantification.
- This method holds significant potential for advancing experimental and clinical diagnostics.
