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Updated: Sep 23, 2025
Author Spotlight: Fluorescence-Based Quantification of Mitochondrial Membrane Potential and Superoxide Levels Using Live Imaging in HeLa Cells
Published on: May 12, 2023
Mitochondria-targeted fluorescent probe for visualization of exogenous and endogenous methylglyoxal in living cells
Zhiming Wang1, Yaye Bian1, Chang Liu1
1Tianjin Key Laboratory for Photoelectric Materials and Devices, and Key Laboratory of Display Materials & Photoelectric Devices, Ministry of Education, School of Materials Science & Engineering, Tianjin University of Technology, Tianjin, 300384, China.
Abstract:
An activatable mitochondria-targeted fluorescent probe Hcy-OPD was synthesized for the detection of methylglyoxal (MGO). For the introduction of a preorganized isopropylamino group on the aromatic o-diamine framework to regulate the hindrance effect, Hcy-OPD showed high selectivity and sensitivity (0.22 μM) for monitoring MGO. The probe can be applied successfully in the imaging of exogenous and endogenous MGO in living cells.
