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Updated: Sep 22, 2025

CRISPR-Cas9-Mediated Genome Editing in the Filamentous Ascomycete Huntiella omanensis
Published on: June 9, 2020
CRISPR/Cas-Mediated Genome Editing of Streptomyces
Lee Ling Tan1, Elena Heng1, Nadiah Zulkarnain1
1Molecular Engineering Lab, Institute of Molecular and Cell Biology, A*STAR, Singapore, Singapore.
Abstract:
Streptomyces are an important source and reservoir of natural products with diverse applications in medicine, agriculture, and food. Engineered Streptomyces strains have also proven to be functional chassis for the discovery and production of bioactive compounds and enzymes. However, genetic engineering of Streptomyces is often laborious and time-consuming. Here we describe protocols for CRISPR/Cas-mediated genome editing of Streptomyces. Starting from the design and assembly of all-in-one CRISPR/Cas constructs for efficient double-strand break-mediated genome editing, we also present protocols for intergeneric conjugation, CRISPR/Cas plasmid curing, and validation of edited strains.
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