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Updated: Sep 21, 2025

CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.
Published on: December 11, 2020
The Cbr-DPY-10(Arg92Cys) modification is a reliable co-conversion marker for CRISPR/Cas9 genome editing in
Itai Antoine Toker1, Oliver Hobert1,2
1Columbia University, Department of Biological Sciences.
Abstract:
The maturation of genome editing techniques dramatically broadens the range of organisms amenable to mechanistic investigation. Caenorhabditis briggsae is a nematode species related to C. elegans and a favored target for comparative studies. Here, we expand the repertoire of co-conversion markers to facilitate the screening and isolation of CRISPR/Cas9-edited lines in C. briggsae . Similar to its homologous C. elegans mutation, Cbr-dpy-10(Arg92Cys) is phenotypically easy to detect in its heterozygous form and is distinguishable from other combinations of Cbr-dpy- 10 alleles, a valuable feature for the reliable isolation of marker-free CRISPR/Cas9-edited animals.
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