Pro-Angiogenic Effects of Natural Antioxidants Extracted from Mango Leaf, Olive Leaf and Red Grape Pomace over
Ismael Sánchez-Gomar1,2, Josefa Benítez-Camacho1,2, Cristina Cejudo-Bastante3,4
1Biomedicine, Biotechnology and Public Health Department, University of Cadiz, 11002 Cadiz, Spain.
Abstract:
Cardiovascular diseases remain the leading cause of death worldwide, mainly triggered by the formation of atherosclerotic plaques that reduce blood flow. Angiogenic cell therapy based on endothelial colony forming cells (ECFCs) constitutes a promising alternative to promote vascular revascularization; however, under the oxidative environment that prevails in ischemic areas, these cells become impaired. Thus, it is necessary to investigate strategies to enhance their regenerative properties. Antioxidant substances, such as polyphenols, have been shown to be useful for this purpose. In the current study we evaluated the potential of mango leaves, olive leaves and red grape pomace extracts, rich in polyphenols, to promote ECFC reparative effects. For this, aqueous and ethanolic extracts of the aforementioned raw materials were obtained by pressurized liquid extraction (PLE). After evaluating the polyphenol content and the antioxidant activity, in vitro assays were carried out, and we found that ethanolic extracts at low concentrations improved angiogenic capacities of ECFCs and reduced proliferation, apoptosis, and the inflammatory response of these cells. Overall, mango leaves ethanolic extract provided the most promising results, but all three extracts ameliorated the functionality of ECFCs.
Insights
Plant extracts rich in polyphenols can enhance endothelial colony forming cells (ECFCs) for cardiovascular repair. Mango, olive, and grape extracts improved ECFC function, offering a promising therapeutic strategy.
Area of Science:
- Cardiovascular research
- Cell biology
- Natural product chemistry
Background:
- Cardiovascular diseases are a leading global cause of death, often linked to atherosclerosis.
- Endothelial colony forming cells (ECFCs) show promise for vascular repair but are impaired by oxidative stress in ischemic conditions.
- Enhancing ECFC regenerative properties is crucial for effective therapeutic strategies.
Purpose of the Study:
- To investigate the potential of polyphenol-rich extracts from mango leaves, olive leaves, and red grape pomace to improve ECFC function.
- To assess the impact of these natural extracts on ECFC angiogenic capacity, proliferation, apoptosis, and inflammatory response.
Main Methods:
- Pressurized liquid extraction (PLE) was used to obtain aqueous and ethanolic extracts from mango leaves, olive leaves, and red grape pomace.
- Polyphenol content and antioxidant activity of the extracts were evaluated.
- In vitro assays were performed to assess the effects of the extracts on ECFCs.
Main Results:
- Ethanolic extracts, particularly at low concentrations, significantly improved the angiogenic capacities of ECFCs.
- These extracts reduced ECFC proliferation, apoptosis, and inflammatory responses.
- Mango leaf ethanolic extract demonstrated the most promising results, though all tested extracts ameliorated ECFC functionality.
Conclusions:
- Polyphenol-rich plant extracts, especially from mango leaves, can enhance ECFC therapeutic potential for cardiovascular applications.
- These natural compounds offer a viable strategy to overcome oxidative stress-induced impairment of ECFCs in ischemic environments.
- Further research into these extracts could lead to novel regenerative therapies for cardiovascular diseases.
More Related Videos
06:12HUVEC Tube-formation Assay to Evaluate the Impact of Natural Products on Angiogenesis
Published on: June 24, 2019
08:43Isolation of Endothelial Progenitor Cells from Healthy Volunteers and Their Migratory Potential Influenced by Serum Samples After Cardiac Surgery
Published on: February 14, 2017
Related Concept Videos
01:24Regulation of Angiogenesis and Blood Supply
01:10Mechanism of Angiogenesis