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Two-Step Reverse Transcription Droplet Digital PCR Protocols for SARS-CoV-2 Detection and Quantification

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Published on: March 31, 2021

Strategies for Scaling up SARS-CoV-2 Molecular Testing Capacity.

Sanchita Das¹, Karen M Frank¹

¹Department of Laboratory Medicine, National Institutes of Health Clinical Center, Bethesda, MD 20892, USA.

Clinics in Laboratory Medicine

|May 31, 2022

Summary

Scaling up severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) testing was vital during the COVID-19 pandemic. Laboratories utilized pooled testing and automation to increase capacity while ensuring high individual test performance.

Keywords:

Automation Capacity Molecular testing Pooling RT-PCR, SARS-CoV-2

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Area of Science:

Virology
Public Health
Laboratory Science

Background:

The COVID-19 pandemic necessitated a rapid scale-up of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) testing.
Maintaining clinical operations and societal functions depended on widespread diagnostic capabilities.

Purpose of the Study:

To review cutting-edge strategies for expanding SARS-CoV-2 testing capacity.
To evaluate methods that maintained high individual test performance during mass testing efforts.

Main Methods:

Review of laboratory strategies including pooled testing.
Analysis of automation in high-throughput diagnostic workflows.
Examination of novel approaches to SARS-CoV-2 detection.

Main Results:

Pooled testing and automation were key to meeting unprecedented demand for SARS-CoV-2 detection.
Various innovative methods were employed to enhance testing throughput.
Strategies focused on balancing speed and accuracy in diagnostic testing.

Conclusions:

Effective scaling of SARS-CoV-2 testing relied on strategic implementation of pooled testing and automation.
Continued innovation in diagnostic technologies is crucial for pandemic preparedness.
Maintaining high-quality testing is paramount even during public health emergencies.