Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Spatially resolved profiling of extracellular vesicles in tissues with Spatial-EV-seq.

Nature biotechnology·2026

Same author

Lysosome-Targeting Chimeras (LYTACs): From Modular Design Principles to Diverse Therapeutic Applications.

Chemistry (Weinheim an der Bergstrasse, Germany)·2026

Same author

Cyclic transformation of stable/metastable nucleic acid structures enables dynamic monitoring of ATP in living cells.

Chemical science·2026

Same author

Dual-Spatially Confined Assembly of DNA Nanowall Stiffens Tumor Cells to Enhance Adoptive T-Cell Immunotherapy.

Journal of the American Chemical Society·2026

Same author

Tracing Tumor-Derived Extracellular Vesicle Matrix Metalloproteinase 14 Using Dual-Target Orthogonal Barcoding-Based Microscale Thermophoretic Assays.

ACS nano·2026

Same author

RENAL-CHIP: Rejection Evaluation via Non-Invasive Analysis of Circulating Podocytes With Herringbone-Chip Isolation Platform.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026

Same journal

The ACS at 150: The History of Analytical Chemistry Publications and a Century of Progress.

Analytical chemistry·2026

Same journal

Machine Learning-Enabled Image Analysis of Complex Chemical Mixtures: Synthetic Urine Droplets as a Test System.

Analytical chemistry·2026

Same journal

H<sub>2</sub>O<sub>2</sub>/Viscosity Tandem-Locked Fluorescent Probes Based on an In Situ Fluorophore Synthesis Strategy for Colitis Imaging and Diagnosis.

Analytical chemistry·2026

Same journal

TopoStitcher: A Geometric-Topological Structure-Guided Stitching Framework for Single-Molecule Localization Microscopy.

Analytical chemistry·2026

Same journal

Noninvasive SERS Immunosensing of Tyrosinase for Melanoma Monitoring via Microneedle Sampling Integrated with Satellite-Structured Bifunctional Nanozymes.

Analytical chemistry·2026

Same journal

Label-Free Electrochemical CRISPR Platform Gated by Allosteric Transcription Factors for Ultrasensitive Small-Molecule Detection.

Analytical chemistry·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Sep 20, 2025

Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles

Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles

Published on: March 13, 2017

DNA-Programmed Orientation-Ordered Multivalent Microfluidic Interface for Liquid Biopsy.

Jiao Peng¹, Yilong Liu¹, Rui Su²

¹MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, State Key Laboratory of Physical Chemical of Solid Surfaces, Key Laboratory for Chemical Biology of Fujian Province, Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.

Analytical Chemistry

|June 7, 2022

Summary

This summary is machine-generated.

This study introduces a novel DNA nanostructure strategy for precisely orienting aptamers on microfluidic chips. This enhances circulating tumor cell capture and release for improved liquid biopsy diagnostics.

More Related Videos

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

Published on: September 10, 2014

Microfluidic Applications for Disposable Diagnostics

Microfluidic Applications for Disposable Diagnostics

Published on: February 3, 2008

Related Experiment Videos

Last Updated: Sep 20, 2025

Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles

Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles

Published on: March 13, 2017

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

Published on: September 10, 2014

Microfluidic Applications for Disposable Diagnostics

Microfluidic Applications for Disposable Diagnostics

Published on: February 3, 2008

Area of Science:

Biotechnology
Nanotechnology
Medical Diagnostics

Background:

Aptamer-functionalized microfluidic devices show promise for liquid biopsies.
Previous designs often overlook aptamer orientation, limiting binding efficiency.
Optimizing aptamer arrangement is crucial for enhanced affinity and accessibility.

Purpose of the Study:

To develop a DNA nanostructure-programmed strategy for precise aptamer orientation and valency control on microfluidic interfaces.
To enhance the capture and release of circulating tumor cells (CTCs) using this novel approach.
To demonstrate the clinical utility of the developed aptamer-based microfluidic chip for leukemia detection and downstream analysis.

Main Methods:

Utilized a DNA nanostructure-programmed strategy to create an ordered aptamer configuration on a microfluidic interface (CDN-Apt-Chip).
Evaluated the synergistic effect of ordered orientation and multivalent aptamer configuration on binding affinity towards CTCs.
Applied the CDN-Apt-Chip for isolating CTCs from leukemia patient blood, discriminating patients from healthy volunteers, and enabling non-destructive cell release.

Main Results:

The ordered aptamer orientation and multivalent configuration significantly increased binding affinity for CTCs.
Successfully isolated CTCs from peripheral blood of T-cell leukemia patients.
Demonstrated the ability to discriminate T-cell leukemia patients from healthy individuals.
Achieved non-destructive release of captured CTCs using nuclease treatment.
Confirmed compatibility of released CTCs with downstream T-cell receptor sequencing.

Conclusions:

The DNA nanostructure-programmed strategy offers precise control over aptamer orientation and valency on microfluidic chips.
This approach enhances CTC capture and release, advancing aptamer-based liquid biopsy.
The CDN-Apt-Chip represents a new paradigm for interface regulation in microfluidic devices, facilitating clinical translation.