Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Elevated Body Mass Index Associated With Uterine Fibroids: A Retrospective Cohort Study With Bioinformatic Validation.

The journal of obstetrics and gynaecology research·2026

Same author

A general and green approach of pH-sensitive ZIF-67 for encapsulating active substances.

Smart molecules : open access·2026

Same author

Instability in sleep architecture and intermittent hypoxia underlie cognitive impairment in patients with obstructive sleep apnea.

Sleep & breathing = Schlaf & Atmung·2026

Same author

Differential cytotoxic effect of polybrene on distinct glioblastoma subtypes.

Neuroscience·2026

Same author

The Impact of Physician Collaboration on Online Medical Team Effectiveness: Evidence from a Telemedicine Platform.

Journal of health communication·2026

Same author

Asymmetric π-Bridge Strategy Enables High-Sensitivity Flexible NIR Organic Photodetectors for In Situ Ultra-Trace Water Pollutant Detection.

Small (Weinheim an der Bergstrasse, Germany)·2026

Same journal

Gaussian-modulated continuous-variable quantum key distribution over 60 km fiber using an integrated silicon photonic receiver.

Optics letters·2026

Same journal

E2E-OCT: end-to-end joint learning model using optical coherence tomography images for vocal cord leukoplakia diagnosis.

Optics letters·2026

Same journal

Holographic generation of panoramic 3D scenes by concave ellipsoidal mirror reflection.

Optics letters·2026

Same journal

Dual-pilot phase recovery with pair-wise maximum-ratio combining for coherent PONs.

Optics letters·2026

Same journal

Mapping the whispering gallery modes of a CaF<sub>2</sub> disk resonator with half-tapered fibers to estimate the fundamental mode volume.

Optics letters·2026

Same journal

Quantitative estimation of deep-subwavelength scale via dark-field scattering axial energy concentration decay profiles.

Optics letters·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Sep 7, 2025

Demonstration of a Hyperlens-integrated Microscope and Super-resolution Imaging

Demonstration of a Hyperlens-integrated Microscope and Super-resolution Imaging

Published on: September 8, 2017

Deformable mirror based optimal PSF engineering for 3D super-resolution imaging.

Shuang Fu, Mengfan Li, Lulu Zhou

|June 16, 2022

Summary

This summary is machine-generated.

We developed a new method for engineering point spread functions (PSFs) using deformable mirrors (DMs) for 3D super-resolution imaging. This device-specific optimization improves imaging performance over conventional methods.

More Related Videos

Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform

Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform

Published on: February 12, 2014

Measurement of Scattering Nonlinearities from a Single Plasmonic Nanoparticle

Measurement of Scattering Nonlinearities from a Single Plasmonic Nanoparticle

Published on: January 3, 2016

Related Experiment Videos

Last Updated: Sep 7, 2025

Demonstration of a Hyperlens-integrated Microscope and Super-resolution Imaging

Demonstration of a Hyperlens-integrated Microscope and Super-resolution Imaging

Published on: September 8, 2017

Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform

Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform

Published on: February 12, 2014

Measurement of Scattering Nonlinearities from a Single Plasmonic Nanoparticle

Measurement of Scattering Nonlinearities from a Single Plasmonic Nanoparticle

Published on: January 3, 2016

Area of Science:

Optical microscopy
Super-resolution imaging
Biophysics

Background:

Point spread function (PSF) engineering encodes molecular properties into light patterns.
Deformable mirrors (DMs) are used for PSF engineering in fluorescence detection due to low photon loss.
DM calibration is crucial for precise wavefront control in PSF design.

Purpose of the Study:

To develop a framework for optimizing PSFs specific to a deformable mirror (DM) for 3D super-resolution imaging.
To generate device-specific optimal PSFs that account for the physical limitations of the DM.
To compare the performance of DM-specific optimal PSFs against conventional PSFs.

Main Methods:

Developed a computational framework for PSF engineering tailored to DM characteristics.
Generated two novel PSFs with extended depth of field using the developed framework.
Performed theoretical calculations and experimental validations to assess PSF performance.

Main Results:

The developed framework successfully generated device-specific optimal PSFs.
The new PSFs demonstrated performance comparable to or exceeding conventional astigmatism and tetrapod PSFs.
Both theoretical and experimental results confirmed the superior performance of the DM-specific optimal PSFs.

Conclusions:

Optimizing PSFs considering the physical behavior of the phase modulator (DM) is essential for advanced imaging.
The developed framework enables the creation of superior PSFs for 3D super-resolution microscopy.
This approach enhances imaging resolution and accuracy by leveraging device-specific optical properties.