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Updated: Sep 7, 2025

Lens-free Video Microscopy for the Dynamic and Quantitative Analysis of Adherent Cell Culture
Published on: February 23, 2018
Snapshot hyperspectral light field tomography
Qi Cui1, Jongchan Park1, Yayao Ma1
1Department of Bioengineering, University of California Los Angeles, Los Angeles, California 90095, USA.
Abstract:
We present snapshot hyperspectral light field tomography (Hyper-LIFT), a highly efficient method in recording a 5D (x, y, spatial coordinates; θ, φ, angular coordinates; λ, wavelength) plenoptic function. Using a Dove prism array and a cylindrical lens array, we simultaneously acquire multi-angled 1D en face projections of the object like those in standard sparse-view computed tomography. We further disperse those projections and measure the spectra in parallel using a 2D image sensor. Within a single snapshot, the resultant system can capture a 5D data cube with 270 × 270 × 4 × 4 × 360 voxels. We demonstrated the performance of Hyper-LIFT in imaging spectral volumetric scenes.

