Split G-Quadruplexes Enhance Nanopore Signals for Simultaneous Identification of Multiple Nucleic Acids

Area of Science:

• Molecular Biology
• Nanotechnology
• Biotechnology

Background:

• Split G-quadruplex (GQ) assemblies offer potential for base-pair specific nucleic acid target identification.
• DNA nanostructures can serve as carriers for molecular probes.
• Solid-state nanopore sensing provides a platform for analyzing molecular structures.

Purpose of the Study:

• To combine split G-quadruplex (GQ) assemblies on DNA nanostructures with nanopore sensing for parallel nucleic acid detection.
• To develop a programmable and sensitive biosensing platform for identifying multiple nucleic acid targets.

Main Methods:

• Designing DNA nanostructures (carriers) functionalized with split G-quadruplex (GQ) probes.
• Utilizing a solid-state nanopore sensing platform for molecular structure analysis.
• Implementing a parallel assay for detecting multiple nucleic acid sequences simultaneously.

Main Results:

• Demonstrated the detection of multiple target sequences from different viruses.
• Achieved low crosstalk between different detected sequences.
• Showcased enhanced nanopore signals upon split GQ formation in the presence of target nucleic acids.

Conclusions:

• The developed DNA-based nanopore sensing platform is programmable and effective for biosensing.
• This approach shows promise for sensitive and specific detection of multiple nucleic acid targets.
• The combination of split GQ assemblies and DNA nanostructures offers a versatile biosensing strategy.