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Decrease in Aflatoxin M1 Concentration in Milk during Cholesterol Removal by Application of β-Cyclodextrin.

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Beta-cyclodextrin effectively reduces carcinogenic aflatoxin M1 in milk during cholesterol removal. This simple, inexpensive method significantly improves milk safety by lowering aflatoxin M1 levels.

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Area of Science:

  • Food Science
  • Food Safety
  • Analytical Chemistry

Background:

  • Aflatoxin M1 (AFM1) is a carcinogenic mycotoxin found in milk, posing a significant public health risk.
  • Chronic exposure to AFM1 affects a substantial portion of the global population.
  • Physicochemical similarities between AFM1 and cholesterol suggest potential for co-removal.

Purpose of the Study:

  • To investigate the efficacy of beta-cyclodextrin (β-CD) in reducing AFM1 concentration in milk.
  • To assess the impact of β-CD-mediated cholesterol removal on AFM1 levels.
  • To evaluate the feasibility of this method for food safety applications.

Main Methods:

  • Milk samples were artificially contaminated with AFM1 (0.20–2.00 µg/kg).
  • Cholesterol removal was performed using 2.0% (w/w) β-cyclodextrin.
  • AFM1 and cholesterol concentrations were measured before and after treatment.

Main Results:

  • Cholesterol levels decreased by 92.3% after β-CD treatment.
  • AFM1 concentration was reduced by 39.1%, reaching 0.53 ± 0.04 µg/kg.
  • The treatment demonstrated significant efficacy in mitigating AFM1 contamination.

Conclusions:

  • Beta-cyclodextrin effectively removes AFM1 from milk alongside cholesterol.
  • This method is easy, inexpensive, and highly applicable for decontaminating milk and dairy products.
  • The findings offer a promising solution to enhance food safety concerning AFM1 presence.