Modification of a Ready-Made High-Voltage Pulse Generator for Non-Thermal Irreversible Electroporation

We present an inexpensive device for non-thermal irreversible electroporation (IRE) manufactured by upgrading an industrial high-voltage pulse generator. In addition to the generator, the experimental hardware-and-software complex (IRE system) contains a microprocessor and a temperature sensor, which enables automatic control the pulse frequency in accordance with the temperature of the object. The IRE system was tested in in vitro and in vivo experiments: effective and safe non-thermal IRE was demonstrated. The developed system is easily reproducible and can be especially useful for demonstration experiments, pioneering research, as well as for teaching the basics of IRE.