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Functional Trachea Reconstruction Using 3D-Bioprinted Native-Like Tissue Architecture Based on Designable
Yingying Huo1,2, Yong Xu3, Xiaodi Wu4
1Department of Plastic and Reconstructive Surgery, Shanghai 9th People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Tissue Engineering, Shanghai, 200011, P. R. China.
Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
|July 26, 2022
Summary
This study presents a novel 3D bioprinting strategy for creating functional trachea tissue. The new method successfully regenerates trachea with native-like structure and function, offering a promising alternative for clinical reconstruction.
Area of Science:
- Biomaterials Science
- Regenerative Medicine
- Tissue Engineering
Background:
- Trachea reconstruction is clinically challenging.
- Current 3D bioprinting lacks suitable bioinks for cartilage and vascularized fibrous tissue.
- Interfacial integration between stiff and soft tissues is a major hurdle.
Purpose of the Study:
- To develop a novel strategy for 3D bioprinting of cartilage-vascularized fibrous tissue-integrated trachea (CVFIT).
- To create tissue-specific bioinks for simultaneous cartilage and vascularized fibrous tissue regeneration.
- To achieve firm interfacial integration between different tissue components.
Main Methods:
- Utilized photocrosslinkable, tissue-specific bioinks for 3D bioprinting.
- Developed cartilage- and fibrous tissue-specific bioinks with synergistic multicomponent effects.
- Employed amidation reaction to enhance interfacial bonding between printed structures.
Main Results:
- Successfully bioprinted tubular trachea analogs with alternant cartilage-fibrous tissue structure.
- Achieved tight integration of ring-to-ring alternant structures.
- Demonstrated satisfactory tissue-specific regeneration and functional recovery in vivo.
Conclusions:
- The developed 3D bioprinting strategy enables the creation of native tissue-like trachea.
- The CVFIT shows potential for clinical applications in trachea reconstruction.
- This approach addresses key challenges in functional trachea regeneration.
