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Related Experiment Videos

New dip-and-read test for determining leukocytes in urine.

A C Skjold, L R Stover, J H Pendergrass

    Clinical Chemistry
    |July 1, 1987
    PubMed
    Summary

    A new reagent strip, LEUKOSTIX, detects white blood cells (leukocytes) in urine using esterase activity. Clinical evaluation showed moderate sensitivity and specificity, comparable to existing tests.

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    Area of Science:

    • Clinical Chemistry
    • Urinalysis
    • Diagnostic Reagents

    Background:

    • Leukocyte detection in urine is crucial for diagnosing urinary tract infections.
    • Existing methods for leukocyte determination can be time-consuming or require specialized equipment.

    Purpose of the Study:

    • To describe and evaluate the performance of a new reagent strip, LEUKOSTIX, for the determination of leukocytes in urine.
    • To compare the efficacy of LEUKOSTIX with a standard method (sediment microscopy) and another commercially available test (CHEMSTRIP LN).

    Main Methods:

    • The LEUKOSTIX test utilizes the esterase activity present in leukocytes to produce a color change.
    • A clinical study involving 867 urine specimens across eight sites was conducted.
    • Results were compared to urine sediment microscopy, with ≥5 cells/high-power field considered positive.

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    Main Results:

    • The LEUKOSTIX reagent strip demonstrated a sensitivity of 76.3% and a specificity of 80.8%.
    • Performance was found to be comparable to the CHEMSTRIP LN leukocyte test.
    • The test relies on visual comparison of the strip's color change to a provided chart.

    Conclusions:

    • LEUKOSTIX offers a viable method for detecting leukocytes in urine based on esterase activity.
    • The reagent strip provides a convenient and accessible option for urinalysis.
    • Further studies may be warranted to optimize sensitivity and specificity for clinical applications.