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Updated: Sep 1, 2025

Characterization of Multi-subunit Protein Complexes of Human MxA Using Non-denaturing Polyacrylamide Gel-electrophoresis
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MYC: a complex problem.

Subhendu K Das1, Brian A Lewis1, David Levens1

  • 1Gene Regulation Section, Laboratory of Pathology, Center for Cancer Research, National Cancer Institute (NCI), Bethesda, MD 20892-1500, USA.

Trends in Cell Biology
|August 13, 2022
PubMed
Summary
This summary is machine-generated.

The MYC protooncogene amplifies transcription but lacks a comprehensive model. This review proposes a

Keywords:
DNA topologyMYCMYC–topoisome complexRNA polymeraseintrinsically disordered proteinsmultistep reactionsprotein complexestopoisomerasetranscription cycle

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Area of Science:

  • Molecular Biology
  • Oncology
  • Gene Regulation

Background:

  • The MYC protooncogene is a key regulator of cellular processes, acting as a universal transcription amplifier.
  • A complete model explaining MYC's complex interactions within transcription and replication machinery is currently lacking.

Purpose of the Study:

  • To review MYC's role as an oncogenic driver and its regulation of gene-regulatory complexes.
  • To propose a novel model for MYC's function and its energetic influence on transcription.

Main Methods:

  • Literature review of MYC's function and interactions.
  • Development of a conceptual 'hand-over model' for MYC trafficking.
  • Analysis of MYC's energetic contribution to the transcription cycle.

Main Results:

  • MYC interacts with a wide array of regulatory and effector complexes.
  • A 'hand-over model' is proposed for the dynamic partitioning and trafficking of unstructured MYC.
  • Unstructured MYC energetically facilitates efficient transcription cycle modulation.

Conclusions:

  • MYC's multifaceted interactions require a dynamic model for understanding its function.
  • The proposed 'hand-over model' offers new insights into MYC's regulatory mechanisms.
  • MYC's energetic role in transcription is crucial for cellular processes and oncogenesis.