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Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

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Updated: Aug 30, 2025

Measurement of Scattering Nonlinearities from a Single Plasmonic Nanoparticle

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Published on: January 3, 2016

Exploiting Dynamic Nonlinearity in Upconversion Nanoparticles for Super-Resolution Imaging.

Chaohao Chen^1,2, Lei Ding³, Baolei Liu⁴

¹Guangdong Engineering and Technology Research Center for Advanced Nanomaterials, School of Environment and Civil Engineering, Dongguan University of Technology, Dongguan 523808, China.

|August 26, 2022

Summary

This summary is machine-generated.

This study enhances super-resolution microscopy by modulating excitation intensity, inducing dynamic optical nonlinearity in upconversion nanoparticles (UCNPs). This method improves spatial resolution for nanoscale imaging without complex equipment.

Keywords:

Upconversion nanoparticles nonlinear optical response super-resolution imaging

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Area of Science:

Nanotechnology
Microscopy
Optics

Background:

Single-beam super-resolution microscopy, or superlinear microscopy, utilizes nonlinear probe responses in confocal microscopy.
This technique offers simplicity, avoiding specialized equipment or beam manipulation.

Purpose of the Study:

To enhance the spatial resolution of single-beam super-resolution microscopy.
To introduce a method for dynamic optical nonlinearity in fluorescent probes.

Main Methods:

Modulating excitation intensity during image acquisition to induce dynamic optical nonlinearity.
Utilizing upconversion nanoparticles (UCNPs) as fluorescent probes.
Applying a weighted finite difference imaging algorithm to extract higher-order fluorescence responses.

Main Results:

Achieved a spatial resolution of 132 nm for resolving single nanoparticles.
Demonstrated improved resolution beyond standard superlinear microscopy.
Successfully applied the technique over a large imaging area.

Conclusions:

The proposed method offers a significant enhancement in super-resolution microscopy.
Dynamic nonlinear fluorescent probes present a promising avenue for advanced nanoscopy.
This approach provides a new strategy for achieving higher resolution in super-resolution imaging.