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Co-Reactant-Mediated Low-Potential Anodic Electrochemiluminescence Platform and Its Immunosensing Application.
Guolin Hong1,2, Canping Su3,2, Mingchun Lai3
1Department of Laboratory Medicine, Xiamen Key Laboratory of Genetic Testing, the First Affiliated Hospital of Xiamen University, School of Medicine, Xiamen University, Xiamen 361003, China.
Analytical Chemistry
|August 31, 2022
Summary
A new electrochemiluminescence (ECL) system using gold nanoclusters (AuNCs) and a novel co-reactant (DIPEA-OH) operates at low potential, enhancing energy efficiency. This system enables sensitive detection of SARS-CoV-2 N protein for potential COVID-19 diagnostics.
Area of Science:
- Electrochemistry
- Nanotechnology
- Biomedical Sensing
Background:
- High-performance anodic electrochemiluminescence (ECL) systems are crucial for expanding applications.
- Co-reactants significantly influence anodic ECL efficiency, with their oxidation being a key step.
- Developing low-triggering potential ECL systems is a key research objective.
Purpose of the Study:
- To develop a novel, high-performance, low-potential electrochemiluminescence (ECL) system using gold nanoclusters (AuNCs).
- To investigate the role of a new co-reactant, 2-(diisopropylamino)ethanol (DIPEA-OH), in enhancing ECL performance.
- To construct a sensitive immunosensor for SARS-CoV-2 N protein detection using the developed ECL system.
Main Methods:
- Synthesis of a bovine serum albumin-protected gold nanocluster (BSA-AuNC) system.
- Modification of triethylamine (TEA) to create a novel co-reactant, DIPEA-OH, with isopropyl substitution and hydroxyl addition.
- Construction of a sandwich-type immunosensor utilizing the BSA-AuNC/DIPEA-OH ECL system for SARS-CoV-2 N protein detection.
Main Results:
- The BSA-AuNC/DIPEA-OH ECL system demonstrated significantly higher energy efficiency at a low potential of 0.75 V.
- Compared to BSA-AuNC/TEA, the ECL intensity increased 22.34-fold and quantum yield (ΦECL) increased 13-fold, reaching 68.17%.
- The developed immunosensor achieved a linear detection range of 0.001-100 ng/mL and a low detection limit of 0.35 pg/mL for SARS-CoV-2 N protein, with good performance in serum samples.
Conclusions:
- The novel DIPEA-OH co-reactant significantly enhances the performance of AuNC-based anodic ECL systems.
- The developed low-potential, high-efficiency ECL system provides a sensitive and selective platform for SARS-CoV-2 N protein detection.
- This work offers a framework for designing advanced ECL co-reactants and expands the potential of ECL sensors in clinical diagnostics, particularly for COVID-19.
