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A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells
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A Perfluoropolyether Microfluidic Device for Cell-Based Drug Screening with Accurate Quantitative Analysis.

Hyun Sun Choi1, Gwang-Noh Ahn1, Gi-Su Na1

  • 1Department of Chemical Engineering, Pohang University of Science and Technology, Pohang 37673, Republic of Korea.

ACS Biomaterials Science & Engineering
|September 12, 2022
PubMed
Summary

New perfluorinated polyether (PFPE) microchips offer precise drug screening. These 3D-printed platforms prevent molecule adsorption, ensuring accurate dose-dependent analyses for pharmaceutical drug development.

Keywords:
3D printingPFPE microchipcell-based drug screeningquantitative analysis

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Area of Science:

  • Biomaterials Science
  • Microfluidics
  • Pharmacology

Background:

  • Conventional poly(dimethylsiloxane) microfluidic chips suffer from hydrophobic molecule adsorption and matrix permeation, compromising drug availability and quantitative analysis accuracy.
  • Accurate dose-dependent drug screening is crucial for evaluating therapeutic implications of chemical compounds in physiological microenvironments.

Purpose of the Study:

  • To develop a novel perfluorinated polyether (PFPE) microchip using digital light processing 3D printing for precise, quantitative drug screening.
  • To assess the performance of PFPE microchips in preventing molecular adsorption and preserving drug concentration for accurate dose-dependent assays.

Main Methods:

  • Fabrication of a PFPE microchip using digital light processing 3D printing.
  • Culture of cells on PFPE microchips to evaluate cell viability, morphology, and proliferation.
  • Testing the PFPE microchip's ability to prevent nonspecific molecular adsorption and matrix permeation.
  • Assessing the preservation of pharmaceutical drug concentration and generation of linear concentration gradients within the PFPE microchannels.

Main Results:

  • Cells cultured on PFPE microchips demonstrated excellent viability, spread morphology, and superior proliferative capability.
  • PFPE microchips, due to low surface energy, significantly inhibited nonspecific molecular adsorption and matrix permeation.
  • The PFPE multibranched channel effectively preserved drug concentration during perfusion, generating a linear concentration gradient.
  • A clear dose-dependent chemotherapeutic effect was observed, validating the platform's quantitative accuracy.

Conclusions:

  • Perfluorinated polyether (PFPE) microchips fabricated via 3D printing serve as a robust platform for quantitative drug screening.
  • The biocompatible and nonadsorbing nature of PFPE microchannels ensures precise concentration-dependent pharmaceutical assays.
  • This technology enhances the accuracy of drug availability and dose-response evaluations in cell-based screening platforms.