Related Experiment Video
Updated: Aug 29, 2025
Yeast As a Chassis for Developing Functional Assays to Study Human P53
Published on: August 4, 2019
P53 Suppressor Gene Tissue Microarray-based Protein Expression Analysis in Meningiomas
Dimitrios Roukas1, Anastasios Kouzoupis2, Despoina Spyropoulou3
1Department of Psychiatry, 417 VA (NIMTS) Hospital, Athens, Greece.
Background/Aim:
Meningiomas represent the main intracranial primary central nervous system (CNS) tumour in adults worldwide. Oncogenes' over-activation combined with suppressor genes' silencing affect negatively the biological behavior of these neoplasms. This study aimed to explore the impact of p53 suppressor gene expression in meningiomas' clinic-pathological features based on a combination of sophisticated techniques.
Materials And Methods:
Fifty (n=50) meningiomas were included in the study, comprising a broad spectrum of histopathological subtypes. An immunohistochemistry assay was applied on tissue microarray cores followed by digital image analysis.
Results:
p53 protein over-expression (high staining intensity levels) was observed in 27/50 (54%) cases, whereas the rest (23/50-/46%) demonstrated moderate to low levels of the protein. p53 over-expression was statistically significantly correlated to the mitotic index of the examined cases (p-value=0.001). Interestingly, the atypical/anaplastic group of histotypes demonstrated the strongest p53 expression rates compared to the others (p-value=0.001).
Conclusion:
p53 overexpression is observed in a broad spectrum of meningiomas. High expression levels lead to an aggressive biological behavior of the malignancy (combined with increased mitotic rates), especially in atypical and anaplastic sub-types that also have a high recurrence rate.
Insights
p53 overexpression is common in meningiomas, a type of brain tumor. High p53 levels correlate with aggressive tumor behavior and higher recurrence rates, particularly in atypical and anaplastic subtypes.
Area of Science:
- Neuro-oncology
- Molecular pathology
- Cancer genetics
Background:
- Meningiomas are the most common primary intracranial CNS tumors in adults.
- Tumorigenesis involves oncogene over-activation and tumor suppressor gene silencing.
- The role of p53 suppressor gene expression in meningioma behavior requires further investigation.
Purpose of the Study:
- To investigate the impact of p53 suppressor gene expression on meningioma clinic-pathological features.
- To analyze p53 protein expression levels in various meningioma subtypes.
- To correlate p53 expression with tumor aggressiveness and mitotic activity.
Main Methods:
- Immunohistochemistry assay on tissue microarray cores from 50 meningiomas.
- Digital image analysis for quantitative assessment of p53 protein expression.
- Inclusion of a diverse range of histopathological subtypes.
Main Results:
- p53 protein overexpression (high staining) observed in 54% of cases (27/50).
- p53 overexpression significantly correlated with increased mitotic index (p=0.001).
- Atypical and anaplastic meningiomas showed the highest p53 expression rates (p=0.001).
Conclusions:
- p53 overexpression is prevalent across various meningioma subtypes.
- High p53 expression is linked to aggressive biological behavior and increased mitotic rates.
- Elevated p53 levels are particularly associated with aggressive atypical/anaplastic meningiomas and their high recurrence rates.
More Related Videos
09:40Characterization of Functionally Associated miRNAs in Glioblastoma and their Engineering into Artificial Clusters for Gene Therapy
Published on: October 4, 2019
07:00Identification of OTX1 and OTX2 As Two Possible Molecular Markers for Sinonasal Carcinomas and Olfactory Neuroblastomas
Published on: February 28, 2019
