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Updated: Aug 28, 2025
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Reverse Yeast Two-hybrid System to Identify Mammalian Nuclear Receptor Residues that Interact with Ligands and/or Antagonists
Published on: November 15, 2013
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Nuclear Receptors and Lipid Sensing.
James L Thorne1, Giorgia Cioccoloni2
1Nutrition, Epigenetics and Cancer Research Group, School of Food Science and Nutrition, University of Leeds, Leeds, UK. j.l.thorne@leeds.ac.uk.
Advances in Experimental Medicine and Biology
|September 15, 2022
Summary
Lipid metabolism, regulated by nuclear receptors, is crucial for health and disease. Understanding these receptors offers new therapeutic targets for cancer and immune disorders.
Area of Science:
- Molecular Biology
- Cell Biology
- Biochemistry
Background:
- Lipid metabolism fluctuations influence diverse cellular functions and are implicated in various diseases.
- Lipid-sensing nuclear receptors play a key role in managing these metabolic changes.
- Recent research highlights the significance of lipids and nuclear receptors in cancer and immuno-oncology.
Purpose of the Study:
- To discuss major lipid-sensing nuclear receptors, including liver X receptors, farnesoid X receptor, and peroxisome proliferator-activated receptors.
- To explore the molecular mechanisms, endogenous ligands, and regulatory pathways of these receptors.
- To examine their therapeutic potential in cancer and immuno-oncology.
Main Methods:
- Review of molecular biology principles governing nuclear receptor function.
- Analysis of endogenous ligand interactions and receptor modulation mechanisms (e.g., miRNA, post-translational modifications).
- Exploration of current research on therapeutic targeting in oncology.
Main Results:
- Lipid-sensing nuclear receptors are central regulators of lipid homeostasis.
- These receptors exhibit complex modulation by endogenous ligands and regulatory pathways.
- Emerging evidence supports their role as viable therapeutic targets in cancer and immuno-oncology.
Conclusions:
- Lipid-sensing nuclear receptors are critical in integrating lipid signaling and cellular functions.
- Targeting these receptors presents promising therapeutic avenues for cancer and immune-related diseases.
- Further research is needed to address existing knowledge gaps and experimental challenges.
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