The coumarin-pyrazole dye for detection of hydrogen sulfide in cells

Fluorescent probes for H₂S are often interfered by other thiols. In this work, a coumarin-pyrazole dye with 2,4-dinitrosulfonyl group was designed for the detection of H₂S. The probe exhibits weak fluorescence in water due to the photo induced electron transfer (PET) by 2,4-dinitrosulfonyl. After the sulfonyl group is cleaved off by H₂S, strong fluorescence appears. The probe can specifically detect H₂S without being interfered by other biological thiols, and shows a wide applicable pH range, low detection and wide detection range. The excellent detection properties of the probe can also be used to detect endogenous and exogenous H₂S in cells. In addition, the probes can be made into portable test paper for the detection of H₂S in solutions and can detect H₂S in different water samples.