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Slow-Off-Rate-Modified Aptamer Labeling for Fluorescence Microscopy and DNA-PAINT
Sebastian Strauss1,2, Ralf Jungmann3,4
1Max Planck Institute of Biochemistry, Martinsried, Germany.
Methods in Molecular Biology (Clifton, N.J.)
|September 26, 2022
Summary
Super-resolution microscopy achieves nanoscale biological visualization. Slow off-rate-modified aptamers (SOMAmers) are introduced as novel labeling reagents to overcome limitations in current super-resolution imaging techniques.
Area of Science:
- Biotechnology
- Microscopy
- Molecular Biology
Background:
- Super-resolution microscopy offers nanoscale visualization of biological processes.
- Current limitations include the need for small affinity reagents.
- Aptamers are nucleic acid alternatives to antibodies.
Purpose of the Study:
- To describe a protocol for using SOMAmers in super-resolution microscopy.
- To address the challenge of limited reagent size in super-resolution imaging.
- To enhance resolution and quantification in DNA-PAINT.
Main Methods:
- Utilizing slow off-rate-modified aptamers (SOMAmers) as labeling reagents.
- Applying SOMAmers for DNA-PAINT super-resolution microscopy.
- Developing a protocol for SOMAmer-based labeling.
Main Results:
- SOMAmers successfully function as labeling reagents for super-resolution microscopy.
- The use of SOMAmers improves resolution and quantification in DNA-PAINT.
- Demonstrated protocol for employing SOMAmers in advanced imaging.
Conclusions:
- SOMAmers are effective small affinity reagents for super-resolution microscopy.
- This protocol enables enhanced nanoscale imaging of biological systems.
- SOMAmers represent a significant advancement for super-resolution techniques like DNA-PAINT.
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