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Structure and Functional Potential of Arctic Sea Sediment Microbiota
William Kurdy1, Galina Yakovleva1, Olga Ilinskaya1
1Institute of Fundamental Medicine and Biology of Kazan (Volga-region) Federal University.
The Journal of General and Applied Microbiology
|November 3, 2022
Summary
Microbial communities in the Laptev Sea are impacted by environmental changes. High-throughput sequencing revealed distinct prokaryotic communities, with the deepest sample showing a unique archaeal dominance and high xenobiotic degradation potential.
Area of Science:
- Microbiology
- Arctic Ecology
- Environmental Science
Background:
- Arctic ecosystems face threats from climate change, pollution, and resource overexploitation.
- Microorganisms are crucial for extreme environments but are understudied.
- Modern sequencing methods are needed to analyze microbial biodiversity and function.
Purpose of the Study:
- To investigate the prokaryotic community composition and diversity in Laptev Sea sediments.
- To analyze the functional potential of these microbial communities.
- To identify potential microbial producers of secreted RNase.
Main Methods:
- High-throughput 16S rRNA sequencing using Illumina MiSeq.
- Analysis of prokaryotic community composition and diversity.
- Functional profiling using the Global Mapper tool.
- Cultivation of bacteria for identifying RNase producers.
Main Results:
- Proteobacteria was the dominant phylum across all samples.
- The deepest sediment sample (185 m) showed a significant increase in archaeal organisms (19%), dominated by Methanosarcinaceace.
- This deepest sample exhibited lower biodiversity and richness indices.
- Functional analysis indicated high potential for xenobiotic degradation in two samples.
- Bacillus and Lysinibacillus genera were identified as potential secreted RNase producers.
Conclusions:
- Microbial community structure in Laptev Sea sediments varies with depth.
- Archaeal abundance and functional capabilities, like xenobiotic degradation, are depth-dependent.
- The identified bacterial genera hold potential for biotechnological applications, such as RNase production.
Keywords:
Arctic ecosystemBiodiversityExtracellular ribonuclease producersHigh-throughput 16S rRNA sequencingLaptev seaMicrobiotaMore Related Videos
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