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Updated: Aug 19, 2025

In Vitro Assay to Study Tumor-macrophage Interaction
Published on: August 1, 2019
Cytotoxic Activity of Macrophages as a Tumor Malignancy Factor
P A Podlesnaya1, O V Kovaleva2, A A Petrenko2
1N. N. Blokhin National Medical Research Center of Oncology, Ministry of Health of the Russian Federation, Moscow, Russia. polina.pod@yandex.ru.
Abstract:
An experimental cell-based model was developed to study antibody-independent cytotoxic activity of macrophages that allows selection of derivative tumor cells resistant to this activity and clarification of the mechanisms responsible for this selection. Cytotoxic macrophages showing antibody-independent cytotoxic activity were obtained. Derivatives of PC3 and H1299 cells resistant to cytotoxic macrophage activity were generated. The main characteristics of the obtained derivative cells were studied. The proposed experimental model can be used as a tool for studying the mechanisms of the development of tumor cells resistance to antibody-independent cytotoxic activity of macrophages.
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