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Growth-Stimulating Effect of Platelet Preparations Obtained by Different Methods on Human Fibroblast Culture
M S Makarov1, M V Storozheva2, N V Borovkova2
1N. V. Sklifosovsky Research Institute of Emergency Medicine, Moscow, Russia. mcsimmc@yandex.ru.
Bulletin of Experimental Biology and Medicine
|November 27, 2022
Summary
Washed platelet lysates effectively stimulate fibroblast growth and viability, unlike other platelet preparations. Proinflammatory cytokines in platelet preparations inversely correlate with cell growth.
Area of Science:
- Biomedical Engineering
- Cell Biology
- Tissue Engineering
Background:
- Platelet-based preparations are utilized for their regenerative potential.
- Different methods of obtaining platelet preparations may influence their biological activity.
- Understanding the optimal use of these preparations is crucial for therapeutic applications.
Purpose of the Study:
- To investigate the differential effects of various platelet preparations on human fibroblast (M-22) growth and viability.
- To determine the optimal dosage and preparation method for stimulating fibroblast proliferation.
- To explore the relationship between cytokine levels in platelet preparations and their impact on cell culture.
Main Methods:
- Culturing human fibroblasts (M-22).
- Preparing and applying different platelet-based products: platelet-rich plasma (PRP) lysates, platelet-poor plasma (PPP) lysates, washed platelet lysates, and activated PRP (ActPRP).
- Assessing cell growth, viability, and structural integrity following treatment with varying volumes (10-500 μl per 10^4 cells) of each preparation.
Main Results:
- Washed platelet lysates (10-500 μl) significantly stimulated fibroblast growth and maintained cell viability.
- Platelet-rich plasma and platelet-poor plasma showed optimal stimulation at 20 μl, while ActPRP was most effective at 500 μl.
- High doses (100-500 μl) of PRP and PPP lysates inhibited fibroblast growth and compromised structural integrity.
- An inverse correlation was observed between cell count and proinflammatory cytokine levels in the tested platelet preparations.
Conclusions:
- Washed platelet lysates represent a promising preparation for promoting fibroblast proliferation and viability.
- The method of platelet preparation critically influences its effect on cell growth, with washed platelets showing superior stimulatory effects.
- Cytokine profiles within platelet preparations play a significant role in modulating fibroblast behavior, highlighting the need for careful preparation and characterization.
