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Updated: Aug 19, 2025

Generation of Human Cardiomyocytes: A Differentiation Protocol from Feeder-free Human Induced Pluripotent Stem Cells
Published on: June 28, 2013
Gene Editing and Human iPSCs in Cardiovascular and Metabolic Diseases
Sebastiano Giallongo1, Oriana Lo Re1,2, Igor Resnick2,3
1Epigenetics, Metabolism and Aging Unit, Center for Translational Medicine, International Clinical Research Center, St'Anne University Hospital, Brno, Czech Republic.
Abstract:
The incidence and the burden of cardiovascular disease (CVD), coronary heart disease (CHD), type 2 diabetes mellitus (T2DM), and the metabolic syndrome are greatly increasing in our societies. Together, they account for 31% of all deaths worldwide. This chapter focuses on the role of two revolutionary discoveries that are changing the future of medicine, induced pluripotent stem cells (iPSCs) and CRISPR/Cas9 technology, in the study, and the cure of cardiovascular and metabolic diseases.We summarize the state-of-the-art knowledge about the possibility of editing iPSC genome for therapeutic applications without hampering their pluripotency and differentiation, using CRISPR/Cas technology, in the field of cardiovascular and metabolic diseases.
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