Related Experiment Video
Updated: Aug 7, 2026
09:52
Histological Quantification to Determine Lung Fungal Burden in Experimental Aspergillosis
Published on: March 9, 2018
Summary
Researchers developed a method to isolate DNA from Aspergillus nidulans. This procedure uses mechanical disruption and proteinase K treatment for pure, high-quality DNA suitable for molecular biology applications.
Area of Science:
- Molecular Biology
- Mycology
- Biochemistry
Background:
- Efficient isolation of high-quality DNA is crucial for molecular studies.
- Aspergillus nidulans is a model organism in fungal genetics and molecular biology.
- Existing DNA isolation methods may not be optimal for preparative scales.
Purpose of the Study:
- To describe a scalable procedure for isolating DNA from Aspergillus nidulans.
- To obtain pure, highly polymerized DNA suitable for downstream applications.
Main Methods:
- Mechanical disruption of lyophilized Aspergillus nidulans.
- Treatment with proteinase K to degrade proteins.
- Sedimentation of the lysate into saturated cesium chloride (CsC1) solution.
Main Results:
- Successful isolation of DNA from Aspergillus nidulans on a preparative scale.
- The isolated DNA was pure and highly polymerized.
- The procedure is robust and reproducible.
Conclusions:
- The described method provides a reliable way to obtain large quantities of pure DNA from Aspergillus nidulans.
- This protocol facilitates advanced molecular and genetic research using this fungus.
More Related Videos
Related Concept Videos
01:34DNA Isolation
DNA from cells is required for many biotechnology and research applications, such as molecular cloning. To remove and purify DNA from cells, researchers use various methods of DNA extraction. While the specifics of different protocols may vary, some general concepts underlie the process of DNA extraction.
01:24DNA Isolation
DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...