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Updated: Aug 17, 2025
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Quantitative Live Cell Fluorescence-microscopy Analysis of Fission Yeast
Published on: January 23, 2012
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Live Cell Imaging of Yeast Golgi Dynamics
Giancarlo Costaguta1, Gregory S Payne2, Lydia Daboussi3
1Department of Neurobiology, David Geffen School of Medicine, University of California, Los Angeles, CA, USA.
Methods in Molecular Biology (Clifton, N.J.)
|December 13, 2022
Abstract:
Fluorescence imaging of live cells allows for the observation of dynamic processes inside cells in real time. Here we describe a strategy to image clathrin-coated vesicle dynamics in a single focal plane at the trans-Golgi network of the yeast Saccharomyces cerevisiae. This method can be readily adapted for live cell imaging of a diverse set of dynamic processes within cells.
