Gold Nanostar Characterization by Nanoparticle Tracking Analysis

We demonstrate the application of nanoparticle tracking analysis (NTA) for the quantitative characterization of gold nanostars (GNSs). GNSs were synthesized by the seed-mediated growth method using triblock copolymer (TBP) gold nanoparticles (GNPs). These GNPs (≈ 10 nm) were synthesized from Au³⁺ (≈ 1 mM) in aqueous F127 (w/v 5%) containing the co-reductant ascorbic acid (≈ 2 mM). The GNS tip-to-core aspect ratio (AR) decreased when higher concentrations of GNPs were added to the growth solution. The AR dependency of GNSs on Au³⁺/Au(seed) concentration ratio implies that growth is partly under kinetic control. NTA measured GNS sizes, concentrations, and relative scattering intensities. Molar absorption coefficients ∼ 10⁹-10¹⁰ M^-1 cm^-1 (ε_400 nm) for each batch of GNSs were determined using the combination of extinction spectra and NTA concentrations for heterogeneous samples. NTA in combination with UV-vis was used to derive the linear relationships: (1) hydrodynamic size versus localized surface plasmon peak maxima; (2) ε_400 nm versus localized surface plasmon peak maxima; (3) ε_400 nm versus hydrodynamic size. NTA for quantitative characterization of anisotropic nanoparticles could lead to future applications, including heterogeneous colloidal catalysis.