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Molecular weight determination of allergen extracts and isolation of allergenic molecules by high-performance liquid
R Wahl1, D Meineke, H J Maasch
1Department of Research and Development of Allergen Preparations, Allergopharma, Joachim Ganzer KG, Reinbek, F.R.G.
Abstract:
The usefulness of size-exclusion high-performance liquid chromatography (HPLC) for the molecular weight determination of allergen extracts as a method of process control was investigated by measuring seven different batches of timothy pollen extracts. Preparative HPLC was used for the isolation of the major allergen Cat 1, from a crude cat hair and skin scraping (CHSS) extract. The chromatograms of the seven batches of the timothy pollen extracts looked very similar, suggesting the same molecular weight distribution in each extract. The major allergen, Cat 1, could be isolated from the crude CHSS extract by preparative HPLC in acceptable purity, determined by crossed radioimmunoelectrophoresis.