Related Experiment Video
Updated: Aug 15, 2025
07:55
Subcellular Fractionation for ERK Activation Upon Mitochondrial-derived Peptide Treatment
Published on: September 25, 2017
7.9K
Giving ERK a jERK from the endosome
Evi Kostenis1, Sergi Bravo1, Jesus Gomeza1
1Department of Molecular, Cellular, and Pharmacobiology, Institute of Pharmaceutical Biology, University of Bonn, Bonn, Germany.
Trends in Pharmacological Sciences
|January 5, 2023
Summary
Endosomes are now recognized as crucial signaling hubs, not just sorting stations. Research highlights their role in the noncanonical activation of ERK by G protein-coupled receptors (GPCRs).
Area of Science:
- Cell Biology
- Molecular Biology
- Biochemistry
Background:
- Endosomes were traditionally viewed as cellular compartments for receptor sorting and degradation.
- Emerging evidence suggests endosomes actively participate in cellular signaling pathways.
Purpose of the Study:
- To investigate the role of endosomes as signaling platforms.
- To explore the noncanonical activation of ERK by G protein-coupled receptors (GPCRs) on endosomes.
Main Methods:
- Utilized advanced microscopy techniques to visualize endosomal signaling.
- Employed biochemical assays to study receptor-mediated signal transduction at endosomes.
Main Results:
- Demonstrated that endosomes function as signaling hubs for GPCRs.
- Confirmed the noncanonical activation of ERK pathways initiated at endosomes.
Conclusions:
- Endosomes are critical sites for GPCR signal transduction.
- This finding redefines the role of endosomes in cellular communication and signaling networks.
Related Concept Videos
01:19Interactions Between Signaling Pathways
6.4K
Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
6.4K
01:22Amplifying Signals via Enzymatic Cascade
8.6K
When a ligand binds to a cell-surface receptor, the receptor's intracellular domain changes shape, which may either activate its enzyme function or allow its binding to other molecules. The initial signal is amplified by most signal transduction pathways. This means that a single ligand molecule can activate multiple molecules of a downstream target. Proteins that relay a signal are most commonly phosphorylated at one or more sites, activating or inactivating the protein. Kinases catalyze...
8.6K
01:15Receptor Downregulation in MVBs
2.1K
Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
The EGFR can initiate signaling pathways that lead to cell proliferation, migration, and differentiation. Overexpression of EGFR stimulates cells to proliferate. Excessive EGFR...
The EGFR can initiate signaling pathways that lead to cell proliferation, migration, and differentiation. Overexpression of EGFR stimulates cells to proliferate. Excessive EGFR...
2.1K
01:24Intracellular Signaling Cascades
47.3K
Once a ligand binds to a receptor, the signal is transmitted through the membrane and into the cytoplasm. The continuation of a signal in this manner is called signal transduction. Signal transduction only occurs with cell-surface receptors, which cannot interact with most components of the cell, such as DNA. Only internal receptors can interact directly with DNA in the nucleus to initiate protein synthesis. When a ligand binds to its receptor, conformational changes occur that affect the...
47.3K
01:39Receptor-mediated Endocytosis
105.0K
Overview
105.0K
01:08Chemical Signaling in the Endocrine System
3.4K
A signaling cascade is a series of events that facilitates the transmission of information within or between cells, culminating in a targeted response in the recipient cell. As chemical messengers, hormones are pivotal in initiating and modulating these intricate signaling cascades based on their solubility.
Lipid-soluble hormones, such as steroid hormones, demonstrate an intracellular action. These hormones traverse cell membranes due to their lipid nature. Once inside the target cell, they...
Lipid-soluble hormones, such as steroid hormones, demonstrate an intracellular action. These hormones traverse cell membranes due to their lipid nature. Once inside the target cell, they...
3.4K